Tris(hydroxymethyl)aminomethane
Tris(hydroxymethyl)aminomethane, commonly referred to as Tris, is a widely used organic compound in biochemistry and molecular biology. Its versatility as a buffering agent makes it indispensable in laboratory settings, particularly for maintaining stable pH levels in various solutions. Below, we provide detailed product specifications, applications, and frequently asked questions to help you better understand this essential chemical.
Product Specifications
- Chemical Name: Tris(hydroxymethyl)aminomethane
- Molecular Formula: C4H11NO3
- Molecular Weight: 121.14 g/mol
- Appearance: White crystalline powder
- Purity: ≥99.9%
- Solubility: Highly soluble in water
- pH Range: 7.0-9.2 (1M aqueous solution at 25°C)
Applications of Tris(hydroxymethyl)aminomethane
| Application | Description |
|---|---|
| Buffer Preparation | Tris is commonly used to prepare buffers for biochemical and molecular biology experiments, such as electrophoresis and protein purification. |
| DNA/RNA Extraction | It serves as a key component in extraction buffers to maintain optimal pH conditions. |
| Cell Culture | Tris buffers are utilized in cell culture media to stabilize pH and support cell growth. |
| Pharmaceuticals | Used in drug formulations to enhance stability and efficacy. |
Tris(hydroxymethyl)aminomethane FAQ
1. What is the shelf life of Tris(hydroxymethyl)aminomethane?
The shelf life of Tris(hydroxymethyl)aminomethane is typically 3-5 years when stored in a cool, dry place, away from moisture and direct sunlight. Ensure the container is tightly sealed to prevent degradation.
2. How do I prepare a Tris buffer solution?
To prepare a 1M Tris buffer solution, dissolve 121.14 g of Tris(hydroxymethyl)aminomethane in 800 mL of distilled water. Adjust the pH using concentrated HCl or NaOH, then bring the final volume to 1 L with water. Filter sterilize if necessary.
3. Is Tris(hydroxymethyl)aminomethane hazardous?
Tris(hydroxymethyl)aminomethane is generally considered safe when handled properly. However, it may cause irritation to the skin, eyes, and respiratory tract. Always wear appropriate personal protective equipment (PPE) such as gloves and goggles when working with this chemical.
Additional Product Features
- High Purity: Our Tris(hydroxymethyl)aminomethane is rigorously tested to ensure minimal impurities, making it ideal for sensitive applications.
- Consistent Performance: Each batch is quality-controlled to meet stringent industry standards.
- Versatile Packaging: Available in various quantities, from small lab-sized containers to bulk orders for industrial use.
For more detailed technical information or to place an order, please contact our customer service team. Tris(hydroxymethyl)aminomethane remains a cornerstone in scientific research, and we are committed to providing the highest quality product to support your work.